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In the middle of the 19th century, an era of technological progress began. Achievements in the field of natural science gave a powerful breakthrough in the development of clinical medicine. The possibilities of the diagnostic direction expanded, the first laboratory studies of biomaterials were carried out. And the more discoveries took place in the field of biochemistry, the more accurate and informative the results of the analyzes became. Also during this period, physical methods of diagnosis began to be actively used: listening and percussion, which doctors still use today.
The works of Professor Botkin have brought many innovations in this area of medicine. At the therapeutic clinic, pathophysiological studies were carried out, which had not been done before. The healing properties of various plants were also studied: adonis, lily of the valley and others, after which they began to be used in medical practice.